How to reconstitute Thymosin beta-4: mixing and storage
A peptide studied for eye conditions and skin wounds; tendon-repair injections lack controlled human evidence.
Also called: Tβ4, TB4, TB-4, Timbetasin, Timbetasin acetate, RGN-259, RGN-137, RGN-352, NL005, a recombinant Tβ4-related product.
Healing and repair peptides · Full-length actin-binding peptide; related recombinant trial formulations.
Comes as: Investigational sterile ophthalmic solution, Investigational skin gel and IV product, Community: lyophilized powder and blends.
Mixed with: Community powder reports use bacteriostatic water. Trial eye drops were supplied sterile and ready to use..
Common vial sizes: 5, 10 mg. Common water amounts: 2 mL.
- Amounts and water volumes describe TB-labelled community products; full-length identity was not confirmed.
- A 5 mg/5 mg BPC/TB blend in 2 mL contains two separate ingredient amounts.
- Home-reconstituted powder is not the sterile RGN-259 eye formulation.
- Trial concentrations must not be converted to mg per dose without the drop volume or applied gel mass.
Before mixing: No universal storage instruction applies across trial formulations and unregulated powder vials.
After mixing: Community users refrigerate mixed powder vials. No validated home-mixed Tβ4 shelf life was found.
Related to Thymosin beta-4
- BPC-157: An experimental repair peptide with mostly animal evidence and a few small human reports.
- TB-500: A short piece of thymosin beta-4 with no proven repair benefit in humans.
- GHK-Cu: A copper-binding skin peptide with small topical studies and untested systemic injections.
- AHK-Cu: A copper peptide used in scalp serums, with laboratory findings and weak patent-only human data.
- ARA-290: Experimental peptide studied for small-fiber nerve damage, with uncertain pain relief and safety.
- Ac-SDKP: An experimental repair peptide with old human studies, but no proven healing dose.
Sources
- 503B Bulk Drug Substances List, FDA · FDA · 2026
- Bulk Drug Substances Used in Compounding Under Section 503A of the FD&C Act, FDA · FDA · 2026
- Cellulitis, NHS · NHS · 2026
- July 23-24, 2026, Meeting of the Pharmacy Compounding Advisory Committee – FDA Briefing Document for TB-500-Related Bulk Drug Substances (TB-500 (free base) and TB-500 acetate), FDA · FDA · 2026
- Medicines, European Medicines Agency · European Medicines Agency · 2026
- MHRA Products | Home, MHRA · MHRA · 2026
- The 2026 Prohibited List, World Anti-Doping Agency · WADA · 2026
- Therapeutic Goods (Poisons Standard—October 2026) Instrument 2026, Australian Government · Australian Government · 2026
- Think twice before injecting peptides bought online: unauthorized products can seriously harm you, Health Canada · Health Canada · 2026
- Thymosin Beta-4, Sport Integrity Australia · 2026
- Anaphylaxis, NHS · NHS · 2023
- Using intramuscular injections in people on oral anticoagulants, NHS Specialist Pharmacy Service · NHS Specialist Pharmacy Service · 2023
- Medical and surgical interventions for neurotrophic keratopathy., Kruoch Z et al. · The Cochrane database of systematic reviews · 2025
- Recombinant human thymosin beta 4 improves ischemic cardiac dysfunction in mice and patients with acute ST-segment elevation myocardial infarction after reperfusion., Zhang Y et al. · Cardiovascular research · 2025
- 0.1% RGN-259 (Thymosin ß4) Ophthalmic Solution Promotes Healing and Improves Comfort in Neurotrophic Keratopathy Patients in a Randomized, Placebo-Controlled, Double-Masked Phase III Clinical Trial., Sosne G et al. · International journal of molecular sciences · 2022
Sources checked: 2026-10-18.
Information only, not medical advice. Peptifacts does not sell anything and does not link to sellers.